mRNA expression analysis
Strain specific analysis of IL6 and IL6R gene expression in B-hIL6/hIL6R mice by RT-PCR. Mouse Il6 and Il6r mRNA were detectable in spleen of wild-type mice (+/+). Human IL6 was detectable only in homozygous B-hIL6/hIL6R mice (H/H) after stimulated with LPS for 3h. Human IL6R mRNA was detectable in homozygous B-hIL6/hIL6R mice but not in wild-type mice with or without LPS stimulation.
Protein expression analysis
Strain specific IL6R expression analysis in homozygous B-hIL6/hIL6R mice by flow cytometry. Splenocytes were collected from WT (+/+) and homozygous B-hIL6/hIL6R mice (H/H), and analyzed by flow cytometry with species-specific anti-IL6R antibody. Mouse IL6R was detectable in WT mice. Human IL6R was exclusively detectable in homozygous B-hIL6/hIL6R mice but not in WT mice.
IL6R expression pattern analysis by IHC
Analysis of spleen leukocytes cell subpopulations in B-hIL6/hIL6R mice
Analysis of spleen T cell subpopulations in B-hIL6/hIL6R mice