B-hCD200/hCD200R mice
| Strain Name | C57BL/6-Cd200tm1(CD200)Cd200r1tm1(CD200R1)/Bcgen | Common Name | B-hCD200/hCD200R mice |
| Background | C57BL/6 | Catalog number | 111888 |
| Aliases |
CD200, MOX1, MOX2, MRC, OX-2, CD200 molecule; CD200R1, CD200R, HCRTR2, MOX2R, OX2R, CD200 receptor 1 |
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模型验证
Protein expression analysis
Analysis of spleen leukocytes cell subpopulations in B-hCD200/hCD200R mice
Analysis of lymph node leukocytes cell subpopulations in B-hCD200/hCD200R mice
Analysis of lymph node T cell subpopulations in B-hCD200/hCD200R mice
Strain specific CD200 expression analysis in homozygous B-hCD200/hCD200R mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD200/hCD200R mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD200 antibody. Mouse CD200 was detectable in WT mice (+/+). Human CD200 was exclusively detectable in homozygous B-hCD200/hCD200R mice (H/H;H/H) but not in WT mice (+/+).
Strain specific CD200R expression analysis in homozygous B-hCD200/hCD200R mice by flow cytometry. Peritoneal exudative macrophages were collected from wild type (WT) mice (+/+) and homozygous B-hCD200/hCD200R mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD200R antibody. Mouse CD200R was detectable in WT mice (+/+). Human CD200R was exclusively detectable in homozygous B-hCD200/hCD200R mice (H/H; H/H) but not in WT mice (+/+).
Analysis of spleen leukocyte subpopulations by FACS.Splenocytes were isolated from female C57BL/6 and B-hCD200/hCD200R mice (n=3, 8 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCD200/hCD200R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD200/hCD200R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Analysis of spleen T cell subpopulations in B-hCD200/hCD200R mice
Analysis of spleen T cell subpopulations by FACS.Splenocytes were isolated from female C57BL/6 and B-hCD200/hCD200R mice (n=3, 8 week-old). Flow cytometry analysis of the splenocytes was performed to assess T cell subsets. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hCD200/hCD200R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD200/hCD200R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in spleen. Values are expressed as mean ± SEM.
Analysis of lymph node leukocyte subpopulations by FACS.Leukocytes were isolated from female C57BL/6 and B-hCD200/hCD200R mice (n=3, 8 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells and NK cells in homozygous B-hCD200/hCD200R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD200/hCD200R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
Analysis of lymph node T cell subpopulations by FACS.Leukocytes were isolated from female C57BL/6 and B-hCD200/hCD200R mice (n=3, 8 week-old). Flow cytometry analysis of the leukocytes was performed to assess T cell subsets. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hCD200/hCD200R mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD200/hCD200R in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in lymph node. Values are expressed as mean ± SEM.
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