Strain specific analysis of GPC1 gene expression in wild type (WT) mice and B-hGPC1 mice by RT-PCR. Mouse Gpc1 mRNA was detectable only in lung of WT mice (+/+). Human GPC1 mRNA was detectable only in homozygous B-hGPC1 mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific GPC1 expression analysis in homozygous B-hGPC1 mice by western blot. Heart was collected from wild type (WT) mice (+/+) and homozygous B-hGPC1 mice (H/H), and analyzed by western blot with anti-GPC1 antibody. Human GPC1 was detectable in homozygous B-hGPC1 mice (H/H).