B-hB7-H3 EL4|BioMice百奥动物

Common name	B-hB7-H3 EL4	Catalog number	310705
Aliases	4Ig-B7-H3, CD276, B7RP-2	Disease	Lymphoma
Organism	Mouse	Strain	C57BL/6N
Tissue types	Lymphoma	Tissue	T lymphocyte

模型验证

Description

The mouse B7h3 gene was replaced by human B7H3 coding sequence in B-hB7-H3 EL4 cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 EL4 cells.

Application

B-hB7-H3 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hB7-H3 EL4 cells. The exogenous promoter and human B7H3 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine B7h3 gene, resulting in a non-functional transcript.

Protein expression analysis

B7-H3 expression analysis in B-hB7-H3 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hB7-H3 EL4 cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 EL4 cells but not wild-type EL4 cells. The 1-H02 clone of B-hB7-H3 EL4 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hB7-H3 EL4 cells. B-hB7-H3 EL4 cells (2x10⁵) and wild-type EL4 cells (2x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Animals with tumor volume over 3000mm³ in the control group were euthanized.

Tumor growth curve of individual mice

B-hB7-H3 EL4 tumor growth of individual mice. B-hB7-H3 EL4 cells (2x10⁵) and wild-type EL4 cells (2x10⁵) were subcutaneously implanted into C57BL/6N mice (female, 6-9-week-old, n=5). As shown in panel, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hB7-H3 EL4 cells. B-hB7-H3 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

Tumor growth curve of individual mice

B-hB7-H3 EL4 tumor growth of individual mice. B-hB7-H3 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 7-week-old, n=6). As shown in panel, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.