Common name |
B-hPSMA MC38 | Catalog number | 310840 |
Aliases | / | Disease | Colon carcinoma |
Organism |
Mouse |
Strain | C57BL/6 |
Tissue types | Colon | Tissue | Colon |
Description
B-hPSMA MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Targeting strategy
Subcutaneous homograft tumor growth of B-hPSMA MC38 cells. B-hPSMA MC38 cells (5x105) and MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). (C) Tumor cells were harvested and assessed for human PSMA expression by flow cytometry. As shown in panel, B-hPSMA MC38 cells were able to establish tumors in vivo but human PSMA was not expressed on the surface of tumor cells.
Subcutaneous homograft tumor growth of B-hPSMA MC38 cells. B-hPSMA MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hPSMA mice (male, 8-week-old, n=4 or 5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hPSMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
B-hPSMA MC38 cells were subcutaneously transplanted into B-hPSMA mice. At the end of the experiment, tumor cells were harvested and assessed for human PSMA expression by flow cytometry. As shown, human PSMA was highly expressed on the surface of tumor cells. Therefore, B-hPSMA MC38 cells can be used for in vivo efficacy studies of novel PSMA therapeutics.