Strain Name |
C57BL/6-Cd55tm1(CD55)Bcgen/Bcgen
|
Common Name | B-hCD55 mice |
Background | C57BL/6 | Catalog number | 111209 |
Related Genes |
CR; TC; DAF; CROM; CHAPLE |
Targeting strategy
Protein expression analysis
Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in T, B cells of wild-type mice. hCD55 was detectable in T, B cells of homozygous mice.
Protein expression analysis
Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in NK and DCs of wild-type mice. hCD55 was detectable in NK and DCs of homozygous mice.
Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in monocytes and macrophages of wild-type mice. hCD55 was detectable in monocytes and macrophages of homozygous mice.
Strain specific CD55 expression analysis in wild-type C57BL/6 and homozygous B-hCD55 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD55 mice (H/H) (n=3, 8-week-old) and analyzed by flow cytometry with species-specific anti-CD55 antibody. mCD55 was detectable in neutrophils of wild-type mice. hCD55 was detectable in neutrophils of homozygous mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from male wild-type C57BL/6 mice and homozygous B-hCD55 mice (n=3, 8-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells, and Tregs in B-hCD55 mice were similar to those in C57BL/6 mice, demonstrating that humanization of CD55 does not change the frequency or distribution of these cell types in spleen. The frequency of leukocyte subpopulations in blood and lymph nodes of B-hCD55 mice was not shown in the data. Values are expressed as mean ± SEM.