Strain specific analysis of C1QA, C1QB, C1QC gene expression in wild-type C57BL/6 mice and homozygous B-hC1Q mice by RT-PCR. Mouse C1qa, C1qb, C1qc mRNA were detectable in thymus of wild-type C57BL/6 mice (+/+). Human C1QA, C1QB, C1QC mRNA were detectable only in homozygous B-hC1Q mice (H/H) but not in wild-type mice.
Protein expression analysis
Strain specific C1Q expression analysis in wild-type C57BL/6 mice and homozygous B-hC1Q mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) (n=4,8-week-old) and homozygous B-hC1Q mice (H/H) (n=4,8-week-old). Mouse C1Q was only detectable in wild-type mice. C1Q was both detectable in wild-type C57BL/6 mice and homozygous B-hC1Q mice. Therefore, it is speculated that this anti-human C1Q antibody is cross-reactive between human and mouse.
Values are expressed as mean ± SEM. Significance was determined by unpaired t-test. ns: non-significant, *p < 0.05, **p< 0.01, ***p < 0.0001.